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2021-08-04 15:17:17
Wonbioi grinder can be used for molecular detection of African classical swine fever virus
Source:Wonbio Biotechnology

It is mentioned in the technical specifications for prevention and control of African classical swine fever (Trial) issued by the Ministry of agriculture


2.5.3.1 rapid detection of etiology: double antibody sandwich enzyme-linked immunosorbent assay, polymerase chain reaction and real-time fluorescent polymerase chain reaction can be used.


Among these methods, PCR or real-time fluorescence quantitative PCR is used as a common and efficient detection method because of its rapidity, simplicity and sensitivity. In the detection of PCR or real-time fluorescence quantitative PCR, the nucleic acid of pig samples collected in advance must be extracted first. The traditional method of nucleic acid extraction by manually grinding pig samples has many limitations: 1. The speed is slow, and a sample takes several minutes; 2. It is potentially dangerous to people. The experimenter has direct contact with the collected samples, which increases the exposure time and opportunity with the samples; 3. The samples are easy to be polluted. During the grinding process, the instruments used and the splashed sample residues may lead to cross contamination between samples, resulting in an increase in the probability of false positives;


The high-throughput tissue grinder developed by Shanghai Wanbai Biotechnology Co., Ltd. can avoid these limitations


Product features


1. High efficiency and good effect


Complete the grinding of 12, 24, 48 and 96 samples within 1 minute; It saves time and labor, and the difference between batches and within batches is small.


2. No cross infection and no loss of samples


The same procedure is set for the same tissue sample to obtain the same grinding effect; The working time is short and the sample temperature rise is small.


The grinding process is completely closed to avoid external pollution and cross infection between samples, and will not cause sample loss.


3. The operation is simple, intuitive and concise


LCD touch screen control, good man-machine interactive interface; The intelligent program controller can set the frequency and time parameters in time.


4. Good stability


Vertical oscillation mode, sufficient grinding and better stability; The vibration during operation is small, and the noise is less than 65dB.


5. Convenient for low temperature operation


The adapter containing the sample can be immersed in liquid nitrogen at the same time, frozen for 1-2 minutes and ground for 15-30 seconds to save liquid nitrogen.


6. Good repeatability


The same procedure is set for the same tissue sample to obtain the same grinding effect; The working time is short and the sample temperature rise is small.


Scope of application


1. Suitable for grinding and crushing various plant tissues, including roots, stems, leaves, flowers, fruits, seeds and other samples;


2. It is suitable for grinding and crushing various animal tissues, including brain, heart, lung, stomach, liver, thymus, kidney, intestine, lymph node, muscle, bone and other samples;


3. Suitable for grinding and crushing fungi, bacteria and other samples;


4. Applicable to grinding and crushing of food and drug composition analysis and detection;


5. It is applicable to the grinding and crushing of volatile samples, including coal, oil shale, wax products and other samples;


6. It is applicable to the grinding and crushing of plastics and polymers, including PE, PS, textiles, resins and other samples.


Flexible configuration


Different adapters can be selected according to customer needs: 2ml, 5ml, 10m, 50ml, etc


Strong technical strength


The technical engineer of the company graduated from the Institute of genetics of famous universities in Shanghai. He has many years of background in molecular biology, cell biology and genetics. He is very proficient in sample preparation, nucleic acid extraction and PCR detection.


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